IKK beta (IKBKB) Rabbit Polyclonal Antibody

SKU AP06526PU-N

IKK beta (IKBKB) rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.; Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 171-220 of Human IKKβ.
• Specificity: This antibody detects endogenous levels of IKK-beta protein.; (region surrounding Leu193)
• Buffer: Phosphate buffered saline (PBS), pH 7.2.; State: Aff - Purified; State: Liquid purified Ig fraction; Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: ~ 86 kDa
• Gene Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• Database Link:
  O14920
• Background: The transcription factor NFkappaB is retained in the cytoplasm in an inactive form by the inhibitory protein IkappaB. Activation of NFkappaB requires that IkappaB be phosphorylated on specific serine residues, which results in targeted degradation of IkappaB. IkappaB kinase alpha (IKKalpha), previously designated CHUK, interacts with IkappaB-alpha and specifically phosphorylates IkappaB-alpha on Serines 32 and 36, the sites that trigger its degradation. IKKalpha appears to be critical for NFkappaB activation in response to proinflammatory cytokines. Phosphorylation of IkappaB by IKKalpha is stimulated by the NFkappaB inducing kinase (NIK), which itself is a central regulator for NFkappaB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKalpha, IKKbeta and IKKgamma (also designated NEMO), and each appear to make essential contributions to IkappaB phosphorylation.
• Synonyms: I-kappa-B-kinase beta, IKK-beta, IKK-B, I-kappa-B kinase 2, IKK2, NFKBIKB

Reference Data