A RAF (ARAF) Rabbit Polyclonal Antibody
| Product Data | |
| Application | IHC, WB |
|---|---|
| Recommended Dilution | Western blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200. |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide, corresponding to amino acids 261-310 of Human Raf. |
| Specificity | This antibody detects endogenous levels of A-RAF protein. (region surrounding Asp297) |
| Buffer | Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide |
| Concentration | 1.0 mg/ml |
| Purification | Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Predicted Protein Size | ~ 68 kDa |
| Gene Name | A-Raf proto-oncogene, serine/threonine kinase |
| Database Link | |
| Background | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue. |
| Synonyms | A-raf-1, ARAF1, PKS, PKS2 |
| Reference Data | |
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