ACAT1 (ACACA) Rabbit Polyclonal Antibody

SKU AP06366PU-N

ACAT1 (ACACA) rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000. ; Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human ACCα. ; The immunogen sequence is around H74, total 14 AA.
• Specificity: This antibody detects endogenous levels of ACC1 protein.
• Buffer: PBS, pH 7.2; State: Aff - Purified; State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).; Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: ~ 265 kDa
• Gene Name: acetyl-CoA carboxylase alpha
• Database Link:
  Q13085
• Background: Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the ratelimiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl- CoA carboxylase in human muscle. ACCα (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACCβ (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACCα is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted Serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the short-term regulatory mechanism of the enzyme. The gene encoding ACCα, which maps to human chromosome 17, encodes the 265 kDa α form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACCβ is homologous to that of ACCα except for an additional peptide of about 150 amino acids at the N-terminus.
• Synonyms: ACACA, ACAC, ACC1, ACCA

Reference Data