PSCA Rabbit Polyclonal Antibody

CAT#: AP06293PU-N

PSCA rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Prostate Stem Cell Antigen antibody (Cat.-No.: AP06293PU-N) in extracts from HepG2 cells.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 41-90 of Human PSCA.
• Specificity: This antibody detects endogenous levels of Prostate Stem Cell Antigen protein (PSCA).
  (region surrounding Val71)
• Formulation: PBS, pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~18 kDa
• Gene Name: prostate stem cell antigen
• Database Link:
  Entrez Gene 8000 Human
  UniProt ID O43653
• Background: Prostate stem cell antigen (PSCA) is a 123 amino acid glycosylated protein that shares homology with the Thy-1/Ly-6 family of glycosyl-phosphatidylinositol GPI)-anchored cell surface antigens. The PSCA gene maps to chromosome 8q24.2 and transcripts are most prevalent in prostate and placenta. The gene encoding c-myc is also located on chromosome 8q and like PSCA, is overexpressed in a large number of prostate cancers. Transcripts for PSCA are also abundant in urothelial tumors, and levels of PSCA transcripts increase in confluent RT112 bladder carcinomas, suggesting that PSCA is a marker for urothelial and gastric tissue carcinogenesis. Among prostate cancer cell surface antigens, PSCA is expressed in over 80% of prostate carcinomas and correlates well to certain prostate cancer phenotypes such as prostate cancer bone metastates.
• Synonyms: Prostate stem cell antigen