Oct4 (POU5F1) Rabbit Polyclonal Antibody

CAT#: AP06256PU-N

Oct4 (POU5F1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Oct-3/4 antibody at 1/500 dilution Lane 1:Hela whole cell lysate Lane 2:Mouse brain tissue lysate Lane 3:Rat brain tissue lysate

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  Immunohistochemistry (IHC) analyzes of OCT3/4 antibody in paraffin-embedded human lung carcinoma tissue

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 200-250 of Human Oct-3/4.
• Specificity: The antibody detects endogenous levels of OCT3/4 protein.
  (region surrounding Lys222)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen. The purity is > 95% (by SDS-PAGE).
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 38 kDa
• Gene Name: POU class 5 homeobox 1
• Database Link:
  Entrez Gene 18999 MouseEntrez Gene 294562 RatEntrez Gene 5460 Human
  UniProt ID Q01860
• Background: Organic cation transporters (OCT) are expressed in the plasma membrane of epithelial cells from a wide range of tissues, where they function in the elimination of ndogenous amines, cationic drugs and other xenobiotics. The structure of OCTs consists of a 12-transmembrane-domain structure and a large extracellular hydrophilic loop. In humans, OCT1 is primarily expressed in the liver while OCT2 is expressed in the kidney. OCT3 is expressed in the placenta, skeletal muscle, prostate, aorta and liver. OCT3, also known as extraneuronal monoamine transporter, is widely expressed in different regions of the brain including the hippocampus, cerebellum and cerebral cortex. OCT3 mediates the uptake of several neuroactive agents, including dopamine, and may play an important role in the disposition of neurotransmitters and cationic neurotoxins in the brain. The genes encoding human OCT1–3 map to a conserved cluster at chromosome 6q26-q27.
• Synonyms: POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding transcription factor 3, Oct-3, Oct-4

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors