FLI1 Rabbit Polyclonal Antibody

SKU AP06124PU-N

FLI1 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.; Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human Fli-1.
• Specificity: This antibody detects endogenous levels of FLI1 protein.; (region surrounding Arg319)
• Buffer: Phosphate buffered saline (PBS), pH 7.2.; State: Aff - Purified; State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE); Preservative: 0.05% Sodium azide
• Concentration: <span>1.0 mg/ml</span>
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Predicted Protein Size: ~ 50 kDa
• Gene Name: Fli-1 proto-oncogene, ETS transcription factor
• Database Link:
  Q01543
• Background: Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element.
• Synonyms: Fli-1 proto-oncogene, ERGB transcription factor, vascular tumor marker

Reference Data