Cyclin F (CCNF) Rabbit Polyclonal Antibody

CAT#: AP06083PU-N

Cyclin F (CCNF) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Cyclin F antibody (Cat.-No.: AP06083PU-N) at 1/500 dilution Lane 1:HEK293T cell lysate Lane 2:Mouse liver tissue lysate Lane 3:Rat liver tissue lysate

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  Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using Cyclin F antibody (Cat.-No. AP06083PU-N).

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution:

  Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.

• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Cyclin F protein (region surrounding Ile772).
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~88 kDa
• Gene Name: cyclin F
• Database Link:
  Entrez Gene 899 Human
  UniProt ID P41002
• Background: Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.
• Synonyms: Cyclin-F, CCNF