GJA1 Rabbit Polyclonal Antibody

CAT#: AP06070PU-N

GJA1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Connexin 43 antibody at 1/500 dilution. Lane 1: HEK293T cell lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat brain tissue lysate

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  Immunohistochemical analysis in paraffin-embedded human breast tissue using GJA1 / Connexin 43 antibody.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Immunofluorescence: 1/50-1/200.
  Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 340-390 of Human Connexin 43.
• Specificity: This antibody detects endogenous levels of GJA1 / Cx43 protein (region surrounding Ser369).
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freeze thaw cycles!
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~43 kDa
• Gene Name: gap junction protein alpha 1
• Database Link:
  Entrez Gene 2697 Human
  UniProt ID P17302
• Background: Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication.
• Synonyms: Gap junction alpha-1 protein, Connexin-43, GJAL