carabin (TBC1D10C) (N-term) Rabbit Polyclonal Antibody

CAT#: AP05728PU-N

carabin (TBC1D10C) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of whole cell lysate from Daudi, Burkitt's lymphoma derived cells probed with Rabbit anti Human Carabin at 1 (A) and 2 (B) ug/ml

• 

  Formalin fixed, paraffin embedded human spleen with Rabbit anti Human Carabin following heat mediated antigen retrieval using sodium citrate buffer (pH6.0).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1 - 2 µg/ml; detects a band of approximately 55kDa in human spleen tissue cell lysates.
  Immunohistochemistry on paraffin sections (requires antigen retrieval).
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A 17 amino acid peptide from near the amino terminus of human Carabin.
• Specificity: This antibody recognises Carabin, also known as TBC1 domain family member 10C (TBC1D10C), a 50 kDa protein belonging to the TBC1 domain family of proteins. This antibody does not cross react with either TBC1D10A or TBC1D10B.
• Formulation: Phosphate buffered saline containing 0.02% Sodium Azide (NaN3)
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for up to one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: TBC1 domain family member 10C
• Database Link:
  Entrez Gene 374403 Human
  UniProt ID Q8IV04
• Background: Carabin differs from other members of the TBC1 domain protein family, which appear to play a role in the regulation of cell growth and differentiation. Studies demonstrate that Carabin is part of a negative regulatory loop for the intracellular TCR signalling pathway and acts as an inhibitor of the Ras signalling pathway. Carabin is also believed to mediate crosstalk between calcineurin and Ras. Carabin is most abundantly expressed in spleen and peripheral blood leucocytes.
• Synonyms: TBC1D10C