Arhgef2 Sheep Polyclonal Antibody

CAT#: AP05081PU-N

Arhgef2 sheep polyclonal antibody, Purified

Product Images

Western blot analysis using anti-Lfc antibody on HEK-293 cell transfected with vector alone (Lane 1), LFC-short (Lane 2) and LFC-long (Lane 3).

Specifications

Product Data

• Applications: IF, IP, WB
• Recommended Dilution: Western Blot: 4 µg/ml. Detects a 118 kDa band in Mouse tissue extracts corresponding to wild-type LFC.
  Immunoprecipitation: 4 µg/ml.
  Immunofluorescence.
• Reactivities: Mouse
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Antibody developed using protein corresponding to amino acids 2 to 447 of the Lfc protein fused to GST.
• Specificity: This antibody recognizes LCF.
• Formulation: Phosphate Buffered Saline and 0.08% Sodium Azide
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: The antibody can be shipped at ambient temperature.
  Store (in aliquots) at -20°C only.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: rho/rac guanine nucleotide exchange factor (GEF) 2
• Database Link:
  Entrez Gene 16800 Mouse
  UniProt ID Q60875
• Background: Lfc is a 118 kDa protein discovered in a screen for oncogenes in NIH 3T3 cells. Lfc is a signaling protein related to Lbc and is part of the Dbl family of proteins. Lfc is a guanine-nucleotide exchange factor (GEF) for the Rho family of small GTPases. Lfc has also been shown to be upstream of the serine-threonine kinase JNK. Lfc has also been shown to mediate the activation of the Rac signaling pathway.
• Synonyms: KIAA0651, LFP40, GEF-H1