DOK1 Rabbit Polyclonal Antibody

CAT#: AP02768PU-S

DOK1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from K562, HL-60 and JK cells using p62Dok antibody

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p62Dok antibody.

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  Immunofluorescence staining of methanol-fixed HeLa cells using p62Dok antibody (Red).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunofluorescence: 1/100 - 1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50 - 1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human p62Dok around the phosphorylation site of tyrosine 398 (E-G-Yp-E-L).
• Specificity: This antibody detects endogenous levels of total p62Dok protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: docking protein 1
• Database Link:
  Entrez Gene 1796 Human
  UniProt ID Q99704
• Background: DOK1 (Downstream of tyrosine kinase 1, or Docking protein 1) is believed to be a mainly cytoplasmic adaptor protein which down-regulates mitogen-activated protein kinase activation, inhibits cell proliferation and transformation, and promotes cell spreading and cell migration. DOK1 appears to be a negative regulator of the insulin signaling pathway.
• Synonyms: Docking protein 1, pp62, p62(dok)