DOK1 pTyr398 Rabbit Polyclonal Antibody

SKU AP02520PU-N

DOK1 pTyr398 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.; Note: Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.; Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Hhuman p62Dok around the phosphorylation site of Tyrosine 398 (E-G-Yp-E-L).
• Specificity: This antibody detects endogenous levels of p62Dok only when phosphorylated at Tyrosine 398.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.; State: Aff - Purified; State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Gene Name: docking protein 1
• Database Link:
  Q99704
• Background: DOK1 (Downstream of tyrosine kinase 1, or Docking protein 1) is believed to be a mainly cytoplasmic adaptor protein which down-regulates mitogen-activated protein kinase activation, inhibits cell proliferation and transformation, and promotes cell spreading and cell migration. DOK1 appears to be a negative regulator of the insulin signaling pathway.
• Synonyms: Docking protein 1, pp62, p62(dok)
• Note: Molecular Weight: 62 kDa

Reference Data