MEK6 (MAP2K6) pSer207 Rabbit Polyclonal Antibody

SKU AP02431PU-N

MEK6 (MAP2K6) pSer207 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.; Immunohistochemistry on Paraffin Sections: 1/50-1/100.; Immunofluorescence: 1/100-1/200.
• Reactivity: Human, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from Human MKK6 around the phosphorylation site of Serine 207 (V-D-Sp-V-A).
• Specificity: This antibody detects endogenous levels of MKK6 only when phosphorylated at serine207.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol; State: Aff - Purified; State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Gene Name: mitogen-activated protein kinase kinase 6
• Database Link:
  P52564
• Background: Mitogen activated protein kinase kinase 6 (MEK6 or MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MEK6, closely related to MEK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38, thus activating it, in response to inflammatory cytokines and environmental stress.; As an essential component of p38 MAP kinase mediated signal transduction pathway, this protein is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
• Synonyms: MAPKK 6, MAPK/ERK kinase 6, SAPKK3, MEK6, MKK6, MAP kinase kinase 6

Reference Data