CDC25C pSer216 Rabbit Polyclonal Antibody

CAT#: AP02408PU-N

CDC25C pSer216 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from 293 cells untreated or treated with serum using cdc25c (pSer216) antibody

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  Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using cdc25c (pSer216) antibody (left) or the same antibody preincubated with blocking peptide (right)

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  Immunofluorescence staining of methanol-fixed HeLa cells using cdc25c (pSer216) antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Serine 216 (S-P-Sp-M-P).
• Specificity: This antibody detects endogenous levels of CDC25C only when phosphorylated at Serine 216.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: cell division cycle 25C
• Database Link:
  Entrez Gene 995 Human
  UniProt ID P30307
• Background: Cdc25C is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It has been highly conserved during evolution and it plays a key role in the regulation of cell division. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Cdc25C is mainly expressed in G2 phase. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
• Synonyms: CDC25