G3BP (G3BP1) pSer232 Rabbit Polyclonal Antibody

CAT#: AP02380PU-N

G3BP (G3BP1) pSer232 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from 239 cells untreated or treated with starvation using G3BP-1 (pSer232) Antibody

• 

  Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue usind G3BP-1 (pSer232) antibody (left) or the same antibody reincubated with blocking peptide (right)

• 

  Immunofluorescence staining of methanol-fixed HeLa cells using G3BP-1 (pSer232) Antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human G3BP-1 around the phosphorylation site of serine 232 (S-S-SP-P-A).
• Specificity: G3BP-1 (phospho-Ser232) antibody detects endogenous levels of G3BP-1 only when phosphorylated at serine 232.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: G3BP stress granule assembly factor 1
• Database Link:
  Entrez Gene 10146 Human
  UniProt ID Q13283
• Background: G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
• Synonyms: G3BP-1, hDH VIII