GSK3 beta (GSK3B) pSer9 Rabbit Polyclonal Antibody

CAT#: AP02304PU-N

GSK3 beta (GSK3B) pSer9 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 3. Western blot analysis of extracts from 293 cells untreated (Lane 1) or treated with Serum (Lane 2) using GSK3B antibody.

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3B antibody.

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  Figure 2. Immunofluorescence staining of methanol-fixed Hela cells showing cytoplsamic staining using GSK3B antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Note: Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide Sequence around the phosphorylation site of Serine 9 (T-T-Sp-F-A) derived from Human GSK3B.
• Specificity: This antibody detects endogenous levels of GSK3β only when phosphorylated at Serine 9.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity-Chromatography using epitope-specific phosphopeptide. Non-phospho specific antibody was removed by chromatography using non-phosphopeptide.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: glycogen synthase kinase 3 beta
• Database Link:
  Entrez Gene 2932 Human
  UniProt ID P49841
• Background: Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. GSK3 has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control and oncogenesis. Two isoforms, alpha (GSK3A; OMIM 606784) and beta, show a high degree of amino acid homology within their catalytic domains. GSK3B is involved in energy metabolism, neuronal cell development and body pattern formation.
• Synonyms: GSK-3 beta, Glycogen synthase kinase-3 beta, GSK3B
• Note: Molecular Weight: 46 kDa