MEK2 (MAP2K2) pThr394 Rabbit Polyclonal Antibody

SKU AP01634PU-N

MEK2 (MAP2K2) pThr394 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA: 1/10000-1/20000.; Western Blot: 1/500-1/1000.; Immunohistochemistry: 1/50-1/200.; Immunoprecipitation: 1/50-1/200.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of MEK2 pThr394 protein.
• Buffer: Phosphate buffered saline (PBS), pH~7.2 containing 15 mM Sodium Azide as preservative.; State: Aff - Purified; State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Gene Name: mitogen-activated protein kinase kinase 2
• Database Link:
  P36507
• Background: Cell proliferation and development are carefully controlled in C. elegans, with each cell following a nearly invariant pattern of differentiation. Vulval development in particular provides a useful model for studying how cell fate is determined. In addition to cell signaling pathways such as Notch and Ras pathways, the establishment of cell polarity and the asymmetric distribution of certain receptors are also critical for proper cell fate determination. In C. elegans, activated Ras initiates a cascade of sequential phosphorylation events, including the protein kinases Raf, MEK, and MAP kinase. The LET-60 Ras-mediated signal transduction pathway controls vulval induction. LIN-45, a member of the Raf family of serine/threonine kinases, and LIN-31, an HNF-3 homologe, act downstream of the Ras protein as necessary components for vulval differentiation. LIN-1 contains an ETS domain and is thought to be a substrate for the ERK subfamily of MAP kinases. The C. elegans proteins MEK-2, a MAP kinase kinase, and SUR-2 also function in the LET-60 Rasmediated vulval induction.
• Synonyms: MAP kinase kinase 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK2, MKK2, MAP kinase kinase 2

Reference Data