MEF2A pThr312 Rabbit Polyclonal Antibody

SKU AP01632PU-N

MEF2A pThr312 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA: 1/20000-1/40000.; Western Blot: 1/500-1/1000.; Immunohistochemistry: 1/50-1/200.; Immunoprecipitation: 1/50-1/200.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of MEF2A protein only when phosphorylated at Thr312.
• Buffer: Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative.; State: Aff - Purified; State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Gene Name: myocyte enhancer factor 2A
• Database Link:
  Q02078
• Background: MEF-2 is a muscle-specific DNA binding protein that recognizes an A+T-rich sequence [CTA (A/T)4 TAG] localized in the control regions of numerous muscle-specific genes. MEF-2 belongs to the MADS (MCM1, Agamous, Deficiens and serum-response factor) box family of transcription factors. The MEF-2 proteins arise from several alternatively spliced isoforms of the MEF-2 gene. MEF-2 expression is ubiquitous, but appears to be preferential in skeletal and cardiac muscle cells. Phosphorylation of different MEF-2C isoforms affects their expression pattern and transactivation function. Big MAP kinase 1 (BMK1) enhances the transactivation activity of MEF-2C by phosphorylating Ser 387. Serum is a potent stimulator of BMK1-induced MEF-2C phosphorylation. p38 MAPK can phosphorylate MEF-2C at positions Ser 387, Thr 293 and Thr 300. Phosphorylation of MEF-2C by either p38 MAPK or ERK5/BMK1 is necessary for Smad-MEF-2 signaling cooperativity.
• Synonyms: MEF2, Myocyte-specific enhancer factor 2A
• Note: Molecular Weight: ~55.0 kDa

Reference Data