ATF2 pSer62/44 Rabbit Polyclonal Antibody

CAT#: AP01529PU-N

ATF2 pSer62/44 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-ATF2 (pSer62/44) antibody in extracts from HeLa cells.

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  Immunohistochemistry (IHC) analyzes of p-ATF2 (pSer62/44) antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA: 1:20000~1:40000.
  Western Blot: 1:500~1:1000.
  Immunohistochemistry: 1:50~1:200.
  Immunoprecipitation: 1:50~1:200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: p-ATF2 (pSer62/44) antibody detects endogenous levels of p-ATF2 protein.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig
• Concentration: 1,0 mg/ml
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: activating transcription factor 2
• Database Link:
  Entrez Gene 1386 Human
  UniProt ID P15336
• Background: Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1, this protein binds the cAMP response element (CRE) and mediates PKA-induced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CREdependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.
• Synonyms: ATF-2, CREB2, CREBP1, Activating transcription factor 2, CRE-BP1, HB16, CREB-2