CYB5R3 Rabbit Polyclonal Antibody

CAT#: AP01486PU-N

CYB5R3 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of CYB5R3 (I169) Antibody at 1/500 dilution. Lane 1: Hela cell lysate. Lane 2: HEK293T cell lysate. Lane 3: Raw264.7 cell lysate.

• 

  Western blot (WB) analysis of CYB5R3 antibody in extracts from HepG2 cells.

• 

  Immunohistochemistry (IHC) analyzes of CYB5R3 antibody in paraffin-embedded human breast tissue.

• 

  Immunohistochemistry (IHC) analyzes of CYB5R3 antibody in paraffin-embedded human placenta tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 141-190 of Human CYB5R3.
• Specificity: CYB5R3 antibody detects endogenous levels of CYB5R3 protein. (region surrounding Ile169)
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 34 kDa
• Gene Name: cytochrome b5 reductase 3
• Database Link:
  Entrez Gene 1727 Human
  UniProt ID P00387
• Background: CYB5R3 (NADH-cytochrome b5, reductase 3, diaphorase 1) is a 301 amino acid protein encoded by the human gene CYB5R3. CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and has two naturally occuring isoforms. Isoform 1 is anchored to the cytoplasmic side of the endoplasmic reticulum membrane and mitochondrion outer membrane,while isoform 2 is the soluble form found in erythrocytes. CYB5R3 is involved in the desaturation and elongation of fatty acids, cholesterol biosynthesis,drug metabolism and, in erythrocytes, methemoglobin reduction. A serine residue at position 117 seems to only be found in persons of African origin. The allele frequency is 0.23 in African Americans. It is not found in Caucasians,Asians, Indo-Aryans or Arabs. This difference seems to have no effect on the enzyme activity.
• Synonyms: NADH-cytochrome b5 reductase 3, B5R, DIA1, Diaphorase-1