ARFGEF2 Rabbit Polyclonal Antibody

CAT#: AP01387PU-N

ARFGEF2 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of BIG2 antibody (Cat.-No.: AP01387PU-N) in extracts from A549 cells.

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  Immunohistochemistry (IHC) analyzes of BIG2 antibody (Cat.-No.: AP01387PU-N) in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: BIG2 antibody detects endogenous levels of BIG2 protein. (region surrounding Leu1527)
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 210 kDa
• Gene Name: ADP ribosylation factor guanine nucleotide exchange factor 2
• Database Link:
  Entrez Gene 10564 Human
  UniProt ID Q9Y6D5
• Background: Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs), a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking. GEPs comprise two major families, large GEPs that are inhibited by brefeldin A (BFA), a protein that effects golgi structure, and a group of smaller GEPs that are insenstive to BFA. Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs of approximately 200 kDa, designated BIG1 and BIG2. Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity. In vivo, BIG1 and BIG2 exist in macromolecular complexes that move between the golgi membranes and cytosol. BIG2 associates with PKA regulatory subunits, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways.
• Synonyms: ARFGEP2, BIG2, Brefeldin A-inhibited GEP2