ID4 (N-term) Rabbit Polyclonal Antibody

SKU AP01280PU-N

ID4 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.; Immunohistochemistry on Paraffin sections: 1/50-1/200.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human Id4.
• Specificity: This antibody detects endogenous levels of ID4 / BHLHB27 protein (region surrounding Ser5).
• Buffer: Phosphate buffered saline (PBS), pH 7.2; State: Aff - Purified; State: Liquid purified Ig fraction; Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: ~ 17 kDa
• Gene Name: inhibitor of DNA binding 4, HLH protein
• Database Link:
  P47928
• Background: Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate the expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins (encoded by Id-related genes) also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (referenced from entrez gene).
• Synonyms: DNA-binding protein inhibitor ID-4, Inhibitor of DNA binding 4

Reference Data