Metabotropic Glutamate Receptor 5 (GRM5) Rabbit Polyclonal Antibody

CAT#: AP01270PU-N

Metabotropic Glutamate Receptor 5 (GRM5) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis with extracts from human A549 cells using mGluR5 / GRM5 antibody.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 30-80 of Human mGluR-5.
• Specificity: This antibody detects endogenous levels of mGluR5 / GRM5 protein (region surrounding Lys56).
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 132 kDa
• Gene Name: glutamate metabotropic receptor 5
• Database Link:
  Entrez Gene 2915 Human
  UniProt ID P41594
• Background: The mGluR proteins (metabotropic glutamate receptors) are members of the G protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic glutamate receptors). The eight currently known mGluR proteins are mediated by two G proteins with opposing regulation of adenylate cyclase pathways. The activities of mGluR-1 and mGluR-5 are mediated by a G protein that activates a phosphatidylinositolcalcium second messenger system and generates a calcium-activated chloride current. The remainder of the eight subtypes of mGluR have an activity mediated by a G protein that inhibits adenylate cyclase activity. mGluR-3, which may interact with GRASP, acts as a receptor for glutamate.
• Synonyms: Metabotropic glutamate receptor 5, GPRC1E, MGLUR5