MUM1 (IRF4) Rabbit Polyclonal Antibody

CAT#: AP01237PU-N

MUM1 (IRF4) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of IRF-4 antibody in extracts from HeLa cells.

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  Western blot (WB) analysis of IRF-4 antibody in extracts from A549 cells.

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  Immunohistochemistry (IHC) analysis of IRF-4 antibody in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin peroxidase.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 262-318 of Human IRF-4.
• Specificity: This antibody detects endogenous levels of IRF-4 protein.
  (region surrounding Tyr294)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 15 mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~52 kDa
• Gene Name: interferon regulatory factor 4
• Database Link:
  Entrez Gene 3662 Human
  UniProt ID Q15306
• Background: Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes.
• Synonyms: Interferon regulatory factor 4, NF-EM5, MUM1, LSIRF