SFRS3 (SRSF3) Rabbit Polyclonal Antibody

CAT#: AP01175PU-N

SFRS3 (SRSF3) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of SRp20 (SFRS3) in extracts from HeLa using SFRS3 antibody.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 120-170 of Human SRp20.
• Specificity: This antibody detects endogenous levels of SFRS3 protein (region surrounding Phe151).
• Formulation: PBS with 0.02% Sodium Azide, 50% glycerol, pH7.2
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE).
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 25 kDa
• Gene Name: serine/arginine-rich splicing factor 3
• Database Link:
  Entrez Gene 6428 Human
  UniProt ID P84103
• Background: Pre-mRNA splicing enhancer elements are short RNA sequences capable of activating weak splice sites in nearby introns that are required for accurate splice site recognition and the control of alternative splicing. Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20, and SF2/ASF. The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/ serine(RS)-rich domain. They are not only essential for constitutive splicing but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing sites. An additional SR-associated protein, p32, tightly associates with SR factors and preferentially inhibits ASF/SF2 functioning as both a splicing enhancer and splicing repressor protein by preventing the stable interaction of ASF/SF2 and RNA.
• Synonyms: SRP20