PAX7 Mouse Monoclonal Antibody [Clone ID: PAX7/497]

SKU AM50306PU-T

PAX7 mouse monoclonal antibody, clone PAX7/497, Purified

Product Data

• Clone Name: PAX7/497
• Application: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.; Flow Cytometry: 0.5-1 µg/million cells. ; Immunofluorescence: 0.5-1 µg/ml.; Western Blotting: 0.5-1 µg/ml. ; Immunoprecipitation: 0.5-1 µg/500 µg protein lysate. ; Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT. ; Positive Control: HeLa, Jurkat, A431, MCF-7, U2OS or NIH/3T3 cells. Stomach or Rhabdomyosarcoma.
• Reactivity: Human, Mouse, Zebrafish
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human PAX7 protein.
• Specificity: The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell- derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveo- lar rhabdomyosarcoma).; Cellular Localization: Nuclear.
• Buffer: 10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 57 kDa
• Gene Name: paired box 7
• Database Link:
  P23759
• Background: The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).
• Synonyms: Pax-7, HUP1

Reference Data