Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: SPM510]

CAT#: AM50133PU-T

Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone SPM510, Purified

Product Images

Western blot in HeLa lysate using Cytokeratin 18 Antibody (Clone SPM510).

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  Formalin-Paraffin colon cancer stained with Cytokeratin 18 Antibody (Clone SPM510).

Specifications

Product Data

• Clone Name: SPM510
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 1-2 μg/ml.
  Western Blotting: 0.5-1 μg/ml.
  Immunoprecipitation: 1-2 μg/500 μg protein lysate.
  Immunohistochemistry on Formalin-fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Recommended Positive Control: MCF-7, HeLa cells, Breast Cancer.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human breast cancer PMC 42 cells.
• Specificity: This Monoclonal Antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
  Cellular Localization: Cytoplasmic.
  Negative Species: Mouse, Rat, Hamster, Cow, Dog, Pig, and Sheep.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 45 kDa
• Gene Name: keratin 18
• Database Link:
  Entrez Gene 3875 Human
  UniProt ID P05783
• Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18