Involucrin (IVL) Mouse Monoclonal Antibody [Clone ID: SY5]

SKU AM33368PU-T

Involucrin (IVL) mouse monoclonal antibody, clone SY5, Purified

Product Data

• Clone Name: SY5
• Application: ELISA, FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating.; Flow Cytometry: 0.5-1 μg/10⁶ cells.; Immunofluorescence: 1-2 μg/ml.; Western Blot: 0.5-1 μg/ml.; Immunoprecipitation: 1-2 μg/500 μg protein lysate.; Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 1-2 μg/ml for 30 minutes at RT.; Staining of formalin-fixed tissues is ENHANCED by digestion with trypsin (or Protease XXV) at 1mg/ml PBS for 5 min at 37°C.; Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
• Reactivity: Canine, Gorilla, Human, Monkey, Porcine
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Involucrin from Human keratinocytes.
• Specificity: It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured Human keratinocytes, this MAb reacts with a 120kDa protein. ; It stains the Involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in Gorilla and owl Monkey, 66kDa in Dog, and a doublet of 105kDa in Pig. ; Its epitope maps between codon 421-568 of Human Involucrin. ; Cellular Localization: Cytoplasmic.
• Buffer: 10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 66-170 kDa
• Gene Name: involucrin
• Database Link:
  P07476
• Background: Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. ; Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
• Synonyms: involucrin

Reference Data