TGF alpha (TGFA) Mouse Monoclonal Antibody [Clone ID: MF9]

SKU AM33298PU-T

TGF alpha (TGFA) mouse monoclonal antibody, clone MF9, Purified

Product Data

• Clone Name: MF9
• Application: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use free BSA Antibody for coaating. ; Western Blotting: 0.5-1.0 µg/ml. ; Flow Cytometry: 0.5-1 µg/million cells. ; Immunofluorescence: 1-2 µg/ml.; Immunoprecipitation: 1-2 µg/500 µg protein lysate. ; Immunohistochemistry on Frozen Sections: 1-2 µg/ml for 30 minutes at RT.; Positive Control: Jurkat or Ramos cells, Heart, Kidney, Pituitary, Breast cancer.
• Reactivity: Bovine, Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A 17-amino acid synthetic peptide from the carboxyl-terminus of Rat TGF-alpha.
• Specificity: This antibody reacts with the C-terminus of TGF alpha and sho ws no cross-reaction with EGF and the neuropeptide synenkephalin. ; The staining with Antibody is completely blocked by the peptide used for raising this antibody. ; Cellular Localization: Cytoplasmic and secreted.
• Buffer: 10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: ~6 kDa
• Gene Name: transforming growth factor alpha
• Database Link:
  P01135
• Background: TGF-alpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
• Synonyms: Transforming growth factor alpha, ETGF

Reference Data