TFF1 (57-84) Mouse Monoclonal Antibody [Clone ID: GE2 (same as R47/94)]

SKU AM33297PU-T

TFF1 (57-84) mouse monoclonal antibody, clone GE2 (same as R47/94), Purified

Product Data

• Clone Name: GE2 (same as R47/94)
• Application: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.; Flow Cytometry: 0.5-1 μg/million cells.; Immunofluorescence: 0.5-1 μg/ml.; Western Blotting: 0.5-1 μg/ml.; Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.; Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1.0 μg/ml for 30 minutes at RT.; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. ; Positive Control: Spent medium of MCF-7 cells, treated with estrogen. Normal stomach. About 60% of breast carcinomas are positive for pS2, especially those that are also positive for estrogen and/or progesterone receptor.
• Reactivity: Human, Monkey
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide of 28 amino acid residues corresponding to (amino acids 57-84) from the C-terminus of Human pS2.
• Specificity: It recognizes a polypeptide of 6.5kDa, identified as pS2 estrogen-regulated protein. Its epitope is localized between aa57-84 of Human pS2 protein.; Cellular Localization: Cytoplasmic.
• Buffer: 10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 6.5 kDa
• Gene Name: trefoil factor 1
• Database Link:
  P04155
• Background: Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60% of breast carcinomas are positive for pS2. ; Staining is cytoplasmic, often with localization to the Golgi apparatus. pS2 is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.
• Synonyms: TFF1, BCEI, Breast cancer estrogen-inducible protein, PNR-2

Reference Data