EPCAM Mouse Monoclonal Antibody [Clone ID: VU-1D9]

CAT#: AM33243RP-N

EPCAM mouse monoclonal antibody, clone VU-1D9, PE

Product Images

Surface staining of HT29 cells using Ep-CAM Antibody (Clone VU-1D9) (red) and isotype control antibody (green).

Specifications

Product Data

• Clone Name: VU-1D9
• Applications: FC
• Recommended Dilution: Flow Cytometry (Cell Surface): Use antibody at 5 µl/test/10⁶ cells (0.2 µg).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Small cell lung carcinoma cells.
• Specificity: This antibody is specific for CD326, also known as Ep-CAM.
  EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
  Cellular Localization: Cell surface.
  Negative Species: Rat.
• Formulation: 10mM PBS
  Label: PE
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: PE
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  This products is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 40-43 kDa
• Gene Name: epithelial cell adhesion molecule
• Database Link:
  Entrez Gene 4072 Human
  UniProt ID P16422
• Background: Epithelial Cell Adhesion Molecule (Ep-CAM), a 38kD cell surface antigen otherwise known as CD326, MOC31 and Ber-EP4. CD326 is a type 1 transmembrane glycoprotein. It is expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells.
• Synonyms: Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18