MHC Class I Heavy Chain (Restricted expression) Mouse Monoclonal Antibody [Clone ID: HC10]

CAT#: AM33035PU-N

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MHC Class I Heavy Chain (Restricted expression) mouse monoclonal antibody, clone HC10, Purified



USD 440.00


Availability*
2 Weeks

Size
    • 100 ug


Product images

Specifications

Product Data
Clone Name HC10
Applications ELISA, EM, FC, IF, IHC, IP, WB
Recommended Dilution Electron microscopy.
ELISA. 
Western Blot.
Immunoprecipitation.
Flow Cytometry. 
Immunocytochemistry.
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Paraffin Sections
.
Recommended Dilutions: 1/100-1/200 for Flow Cytometry and for Immunohistochemistry with avidin-biotinylated Horseradish Peroxidase complex (ABC) as detection reagent, and 1/100-1/1000 for Immunoblotting applications.
Reactivities Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with HLA-B7 and -B40 heavy chains
Specificity This Monoclonal antibody HC10 recognizes HLA class I heavy chains. HC10 reacts mostly with HLA-B and HLA-C heavy chains and some HLA-A (HLA-A10, HLA-A28, HLA-A29, HLA-A30, HLA-A31, HLA-A32, HLA-A33). 
HC10 was raised against free class I heavy chains of HLA antigens to obtain antibodies that would still react with denatured class I antigens, as they occur in Western blotting, conventional light microscopical analysis of Formalin-Fixed, Paraffin-Embedded Sections, and Cryo-Immuno-Electron Microscopy. HC10 indeed retains strong reactivity with free class I heavy chains in Western blots.
HC10 also produces strong reactivity in Immuno-Electron Microscopy. Its use allows the determination of tissue and subcellular distribution of class I antigens.
Formulation PBS
State: Purified
State: Liquid purified IgG fraction
Preservative: 0.09% Sodium Azide
Concentration lot specific
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background The HLA class I gene family is composed of a group of genes whose products encode cell surface glycoproteins of MW 40–45 kDa, associated non-covalently with the beta-2-microglobulin light chain. They include the three polymorphic molecules HLA-A, -B, and -C, which are ubiquitously expressed and which are able to present intracellular peptides to cytotoxic T cells. Three additional class I genes are known, commonly referred to as non-classical or class Ib genes, all highly homologous to the other class I genes and all of which associate with beta-2-microglobulin light chain. In humans, each of the class Ib genes appears to exhibit a distinct pattern of expression in developing and adult tissues. HLA-E transcripts are distributed widely in adult tissues and have also been found in the placenta and fetal liver. In the adult, the presence of HLA-F has been shown in skin, resting T cells, and B cells, whereas its expression during development has been reported in fetal liver and at low levels in placenta and extra-placental tissues. HLA-G was originally thought to be expressed only in certain populations of placental trophoblasts, but low levels have also been found in a variety of human tissues. Recently it was shown that HLA class I expression in breast cancer cells can have a predictive value for chemotherapy response.
Synonyms HLA Class 1, MHC Class 1, Major Histocompatibility complex class I
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.