Syne3 (Alpha) Mouse Monoclonal Antibody [Clone ID: Nsp-3]

SKU AM33013PU-N

Syne3 (Alpha) mouse monoclonal antibody, clone Nsp-3, Purified

Product Data

• Clone Name: Nsp-3
• Application: IF, IHC, WB
• Recommended Dilution: Immunoblotting.; Immunofluorescence. ; Immunohistochemistry on Frozen Sections: Nsp3 is useful for immunohistochemistry on Frozen tissues and Cell cultures fixed with methanol, or 4% paraformaldehyde combined with a permeabilization step of 15' in 0.1% Triton in PBS.; Recommended Dilutions: 1/100-1/1000 with Avidin-Biotinylated horseradish peroxidase Complex (ABC) as detection reagent.
• Reactivity: Mouse
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: GST fusion protein encoding the seven^th spectrin repeat of NSP-3a.
• Specificity: The Nsp3 monoclonal antibody reacts specifically with Nesprin-3a.
• Buffer: PBS; State: Purified; State: Liquid purified IgG fraction; Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freeze-thaw cycles.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Gene Name: spectrin repeat containing, nuclear envelope family member 3
• Database Link:
  Q4FZC9
• Background: Nesprin-3, is a member of the nesprin family and is a transmembrane protein of the outer nuclear membrane (ONM). Like Nesprin-1 and Nesprin-2, Nesprin-3 also contains a COOH-terminal klarsicht/ANC-1/syne (KASH) domain and a series of spectrin repeats. In humans, the full length nesprin-1 and nesprin-2 are giant proteins, containing both an actin-binding domain resulting in molecular masses of respectively 976 and 764 kDa.; These proteins are therefore suggested to be involved in nuclear positioning. Nesprin-3, in contrast to Nesprin-1 and Nesprin-2, lacks the actin-binding domain and cannot bind directly to the cytoskeleton. Nesprin-3 has two isoforms, Nesprin-3a and Nesprin-3b, which differ in the N-terminus. The N-terminus of Nesprin-3a, but not that of Nesprin-3b interacts with the cytoskeletal crosslinker protein Plectin.; Plectin influences the structural organization and integrity of the cytoskeleton and is thought to assist in the mechanical strengthening of the cells. Plectin binds to nesprin-3a and to the intermediate filaments through its C-terminus, suggesting that the interaction between nesprin-3a and plectin links the nucleus to the intermediate filament system. Nesprin-3, like Nesprin-1 and Nesprin-2 is localized in the nuclear envelope.; This localization is due to its interaction with SUN proteins, both SUN-1 and SUN-2, present in the inner nuclear membrane.
• Synonyms: C14orf49

Reference Data