gamma Actin (ACTG1) (Cytopl.) Mouse Monoclonal Antibody [Clone ID: 2A3]

CAT#: AM33003PU-N

gamma Actin (ACTG1) (Cytopl.) mouse monoclonal antibody, clone 2A3, Purified

Product Images

Methanol fixed human dermal fibroblasts immunostained with 2A3 (1/500)

Specifications

Product Data

• Clone Name: 2A3
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA.
  Flow Cytometry.
  Immunoblotting
  Immunocytochemistry on fixed cells.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin-Embedded Sections.
  Recommended Dilutions: 1/100-1/500 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/1000-1/5000 for immunoblotting applications.
• Reactivities: Chicken, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Peptide comprising the N-terminal nonapeptide of γ-cytoplasmic Actin with an acetylated N-terminus coupled to KLH through the cysteine residue.
• Specificity: This 2A3 Monoclonal antibody is highly specific for γ-cytoplasmic Actin (γ-actin 1; encoded by ACTG1).
  It does not cross-react with the γ-actin isoform expressed in the enteric smooth muscle tissues (γ-actin 2; encoded by ACTG2).
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freeze-thaw cycles.
• Stability: Shelf life: One year from despatch.
• Gene Name: actin gamma 1
• Database Link:
  Entrez Gene 71 Human
  UniProt ID P63261
• Background: Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates.
  β- and γ-cytoplasmic actin play crucial roles during various key cellular processes. Whereas β-actin is preferentially localized in stress fibers, circular bundles and at cell-cell contacts, suggesting a role in cell attachment and contraction, γ-actin displays a more versatile organization, according to cell activities. In moving cells, γ-actin is mainly organized as a meshwork in cortical and lamellipodial structures, suggesting a role in cell motility. β- and γ-actin depleted fibroblasts exhibit distinct changes in motility compared with their controls, suggesting a specific role for each isoform in cell locomotion.
• Synonyms: ACT; ACTB; ACTG; DFNA20; DFNA26; Gamma-actin