GPR48 (LGR4) Rat Monoclonal Antibody [Clone ID: BBX-3A8]

CAT#: AM32985PU-N

GPR48 (LGR4) rat monoclonal antibody, clone BBX-3A8, Purified

Product Images

SDS-PAGE analysis of purified BBX-3A8 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified BBX-3A8 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.

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  FACS analysis of BOSC23 cells using BBX-3A8. BOSC23 cells were transiently transfected with an expression vector encoding either LGR4-hum (red curve), LGR4-murine (blue curve) or an irrelevant protein (control transfectant: black curve). Binding of BBX-3A8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained with LGR4- hum and LGR4-mur transfected cells.

Specifications

Product Data

• Clone Name: BBX-3A8
• Applications: ELISA, FC, WB
• Recommended Dilution: Flow cytometry: 1.2 µg/10⁶ cells.
  Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
• Reactivities: Human, Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: cDNA encoding Human LGR4.
• Specificity: Recognizes LGR4 / GPR48.
  Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: leucine-rich repeat containing G protein-coupled receptor 4
• Database Link:
  Entrez Gene 55366 Human
  UniProt ID Q9BXB1
• Background: LGR4 (Leucine-rich repeat- containing G protein-coupled receptor 4) belongs to the superfamily of G protein-coupled receptors (GPCRs). It is a 951 amino acid multi-pass membrane protein and has multiple N-terminal leucine-rich repeats, which are important for interaction with the glycoprotein ligands, and 7 transmembrane domains (1,3). LGR4 is highly expressed in the adult human pancreas but also with moderate levels of expression in placenta, kidney, brain and heart (2). LGR4 functions as an orphan receptor that may be involved in physiologic activities throughout the cell. It is overexpressed in various cancer types and is thought to enhance carcinoma invasiveness and metastasis, suggesting an important role in tumor progression.
• Synonyms: G-protein coupled receptor 48