BCL2 (41-54) Mouse Monoclonal Antibody [Clone ID: 8C8]

CAT#: AM32832PU-S

BCL2 (41-54) mouse monoclonal antibody, clone 8C8, Purified

Product Images

Western blot analysis of Bcl-2 in Human A) HeLa, B) A549 and C) MCF7 lysate using Bcl-2 Antibody (Clone 8C8).

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  Formalin-Fixed, Paraffin-Embedded Human tonsil stained with Bcl-2 Antibody (Clone 8C8). Note cell membrane and cytoplasmic staining.

Specifications

Product Data

• Clone Name: 8C8
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: For coating, Use Antibody without BSA.
  Western blot: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunofluorescence: 1-2 µg/ml.
  Flow Cytometry: 0.5-1µg/10⁶ cells.
  Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min. Epitope unmasking in EDTA (compared to Citrate buffer) is superior.
  Positive Control: Jurkat, K562, HL-60, or HeLa Cells, Tonsil or follicular lymphomas.
• Reactivities: Human, Monkey, Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to amino acids 41-54 of Human Bcl-2
• Specificity: This antibody recognizes a protein of 25-26kDa, identified as the bcl-2-apha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein.
  The Monoclonal 8C8 antibody has been extensively used to monitor the status of Bcl-2 expression in a myriad of normal and cancerous tissue types, developmental stages, as well as in cultured cells.
  Cellular Localization: Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
  Negative Species: Mouse and Rat.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 25-26 kDa
• Gene Name: B-cell CLL/lymphoma 2
• Database Link:
  Entrez Gene 596 Human
  UniProt ID P10415
• Background: Bcl-2 (Bcl2) is the founding member of the Bcl-2 family. Family members have pivotal roles in regulating apoptosis or death signaling pathways through their control of mitochondrial permeability and cytochrome release (reviewed in Anvekar, 2011; Martinou and Youle, 2011). Bcl-2 derives its name from B cell lymphoma 2 where was first found to be highly expressed in follicular lymphomas with 14;18 reciprocal translocations. There are two isoforms, alpha and beta, generated by alternative splicing and differing in their carboxy termini. Human Bcl-2 alpha is a 239 amino acid (aa) protein and human Bcl-2 beta is a 205 aa protein.
  Bcl-2 is over expressed in neoplastic germinal centers of a majority of follicular lymphomas, whereas the normal or hyperplastic germinal centers are primarily negative for Bcl-2 expression. Upregulation has also been described in a number of other types of tumors. Bcl-2 expression is often considered to be a marker of cell death status, and over or high expression has often been tied to anti-apoptotic states, or resistance to death. However, the actual status of vulnerability to death can depend on the balance of other Bcl-2 family members present, their interaction with one another, as well as other factors.
• Synonyms: BCL2, Bcl-2 alpha