GFAP Mouse Monoclonal Antibody [Clone ID: GA5]

SKU AM32829PU-S

GFAP mouse monoclonal antibody, clone GA5, Purified

Product Data

• Clone Name: GA5
• Application: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating.; Western Blot: 0.5-1 µg/ml. ; Flow Cytometry: 0.5-1 µg/10⁶ cells. ; Immunofluorescence: 1-2 µg/ml.; Immunoprecipitation: 1-2 µg/500 µg protein lysate. ; Immunohistochemistry on Cryo Sections: 0.5-1 µg/ml (See also Tobin et. al. for details).; Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.; Recommended Positive Control: Brain or Astrocytoma.
• Reactivity: Bovine, Chicken, Human, Mouse, Porcine, Rabbit, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Glial Fibrillary Protein from Porcine spinal cord
• Specificity: This Monoclonal antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. ; It labels some astrocytes and some CNS ependymal cells but not oligodendrocytes or neurons. ; Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.; Cellular Localization: Cytoplasmic.
• Buffer: 10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A/G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: ~50 kDa
• Gene Name: glial fibrillary acidic protein
• Database Link:
  P14136
• Background: Glial fibrillary acidic protein (GFAP) is a class-III intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. The gene is localized to chromosome 17q21. Alternate splicing of this gene generates several transcript variants encoding three different isoforms.
• Synonyms: Glial Fibrillary Acidic Protein

Reference Data