Chromogranin A (CHGA) Mouse Monoclonal Antibody [Clone ID: CGA414]

SKU AM32816PU-S

Chromogranin A (CHGA) mouse monoclonal antibody, clone CGA414, Purified

Product Data

• Clone Name: CGA414
• Application: ELISA, FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating. ; Flow Cytometry: 0.5-1 µg/10⁶ cells. ; Immunofluorescence: 1-2 µg/ml. ; Western Blot: 0.5-1 µg/ml. ; Immunoprecipitation: 1-2 µg/500 µg protein lysate.; Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. ; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. ; Positive Control:Adrenal gland, bowel, thyroid, pancreas, or pheochromocytoma.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human Chromogranin A protein.
• Specificity: Recognizes Human Chromogranin A. Other species not tested. ; Cellular Localization: Finely granular cytoplasmic.
• Buffer: 10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 68-75 kDa
• Gene Name: chromogranin A
• Database Link:
  P10645
• Background: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. ; Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
• Synonyms: Chromogranin-A, CgA, CHGA, SP-I

Reference Data