EMA (MUC1) Mouse Monoclonal Antibody [Clone ID: 175C5]

CAT#: AM32800PU-N

EMA (MUC1) mouse monoclonal antibody, clone 175C5, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded human breast tissue stained with CD227 / Mucin-1 / MUC1 Antibody at 10 ug/ml after heat-induced antigen retrieval.

Specifications

Product Data

• Clone Name: 175C5
• Applications: ELISA, EM, FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Western Blot.
  Immunofluorescence.
  Immunoprecipitation.
  Electron Microscopy.
  Flow Cytometry.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections (10 µg/ml).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human mammary carcinoma cell line (ZR-75-1).
• Specificity: Shows a high preference for breast carcinomas relative to normal breast epithelium.
  The antibdy reacts with a carcinoma-associated antigen in both adenocarcinomas and squamous cellcarcinomas of different origins.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: mucin 1, cell surface associated
• Database Link:
  Entrez Gene 4582 Human
  UniProt ID P15941
• Background: This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals.
  Mucin 1, cell surface associated (MUC-1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in humans. MUC-1 is a glycoprotein with extensive O-linked glycosylation of its extracellular domain. Mucins line the apical surface of epithelial cells in the lungs, stomach, intestines, eyes and several other organs.
  The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with colon, breast, ovarian, lung and pancreatic cancers.
• Synonyms: MUC-1, PEMT, Episialin, EMA, H23AG, PUM, DF3, CA 15-3