TBX21 Rabbit Monoclonal Antibody [Clone ID: MRQ-46]

CAT#: AM32239SU-N

TBX21 rabbit monoclonal antibody, clone MRQ-46, Supernatant

Specifications

Product Data

• Clone Name: MRQ-46
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections.
  Recommended dilution: 1/100-1/500.
  Positive Control: Tonsil, hairy cell leukemia.
  Staining pattern: Nuclear.
  Preparation and Pretreatment
  1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
  2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) technique in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
  3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG1
• Clonality: Monoclonal
• Formulation: PBS, pH 7.4
  State: Supernatant
  State: Liquid Tissue Culture Supernatant
  Stabilizer: 0.9% BSA
  Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: T-box 21
• Database Link:
  Entrez Gene 30009 Human
  UniProt ID Q9UL17
• Background: T-bet, a T-box transcription factor, is expressed in CD4+ T-lymphocytes committed to T-helper (Th)1 T-cell development from naïve T-helper precursor cells (Thp) and redirects Th2 T cells to Th1 development. Anti-T-bet is a marker of mature T-cells and is expressed at very low levels in Thp cells and is absent in precursor T-lymphoblastic leukemia/lymphoma cells. Scattered small lymphocytes in the interfollicular T-cell zone of reactive lymphoid tissue, including tonsil, lymph node, and spleen exhibited nuclear staining for T-bet, with no T-bet staining observed in germinal centers or mantle or marginal zones.
  T-bet is expressed in a significant subset of B-cell lymphoproliferative disorders, particularly at an early stage of B-cell development (precursor B-cell lymphoblastic leukemia/lymphoblastic lymphoma), and B-cell neoplasms derived from mature B cells, including CLL/SLL, marginal zone lymphoma, and hairy cell leukemia. In contrast, B-cell neoplasms derived from pregerminal center or germinal center B-cells, including mantle cell lymphoma, follicular lymphoma, diffuse large B-cell lymphoma, and Burkitt lymphoma are negative for T-bet. Therefore, anti-T-bet should serve as a useful marker for the diagnosis and subtyping of B-cell and T-cell lymphoproliferative disorders.
• Synonyms: T-bet, TBLYM