GNRHR Mouse Monoclonal Antibody [Clone ID: F1G4]

CAT#: AM32064PU-N

GNRHR mouse monoclonal antibody, clone F1G4, Purified

Specifications

Product Data

• Clone Name: F1G4
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Flow Cytometry.
  Western Blot: 1-2 µg/ml
  Immunohistochemistry on Frozen Sections: The Monoclonal antibody F1G4 specifically stains Human GnRH receptors on frozen tissue of the anterior pituitary.
  The mAb has been found to react with T47D breast carcinoma cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Mice were immunized with a BSA-conjugated peptide corresponding to amino acids 1-29 of Human GnRH receptor extracellular domain.
  Splenocytes were fused with SP2/0 mouse myeloma cells. Stability was established by subcloning four times.
• Specificity: This Monoclonal antibody F1G4 reacts with GnRH receptors in the anterior pituitary.
  F1G4 showed specificity for the synthetic peptide in ELISA and Dot blot.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: gonadotropin releasing hormone receptor
• Database Link:
  Entrez Gene 2798 Human
  UniProt ID P30968
• Background: Gonadotropin Releasing Hormone (GnRH) is down-regulated by hCG and believed to be an autocrine factor that regulates the ovary. The Gonadotropin Releasing Hormone Receptor (GnRHR) is synthesized in the pituitary gland. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinising hormone (LH) as well as follicle-stimulating hormone (FSH). The receptor contains of seven hydrophobic transmembrane domains connected by hydrophillic extracellular, and intracellular loops characteristic of G-protein coupled receptors.
• Synonyms: Gonadotropin-releasing hormone receptor, GnRH-R, GRHR