MRP8 (S100A8) Mouse Monoclonal Antibody [Clone ID: CF-145]

CAT#: AM31838FC-N

MRP8 (S100A8) mouse monoclonal antibody, clone CF-145, FITC

Product Images

S100A8 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Spleen at 1/50 followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

• 

  Cell Source: Human Peripheral Blood Leukocytes labeled with. Percentage of cells stained above control: 99.9%

Specifications

Product Data

• Clone Name: CF-145
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Flow Cytometry: 0.5 µg/1x10⁶ cells.
  Immunofluorescence.
  Western blot.
  Immunohistochemistry on Paraffin Sections: 1/50.
  Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified granulocyte antigen of Human origin.
  Donor: Human leukocytes.
  Fusion Partner: NSO Mouse Myeloma Partner.
• Specificity: This antibody detects Calgranulin A (S100A8).
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG fraction of Ascitic fluid
  Stabilizer: EIA grade BSA to bring total protein concentration to 4-5 mg/ml
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Protein G Chromatography
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: S100 calcium binding protein A8
• Database Link:
  Entrez Gene 6279 Human
  UniProt ID P05109
• Background: Calgranulin A, also known as MRP-8 and S100A8 is a member of the S100 family of proteins containing 2 EF hand (alpha helix, turn, alpha helix structure) calcium binding motifs. S100 proteins are localized in the cytoplasm and /or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation.
  MRP-8 (S100A8) forms a heterodimeric complex with Calgranulin B, (MRP-14, S100A9) in the cytosol of monocyte and neutrophil cell types circulating in peripheral blood. Calgranulin A is found in elevated levels in the serum of cystic fibrosis cases and is also expressed in the skin of patients with psoriasis, eczematous dermatitis and squamous cell carcinoma.
• Synonyms: S100-A8, CAGA, MRP-8, CFAG
• Note: Protocol: Tests Results
  Tissue Distribution by Flow Cytometry Analysis:
  Cell Concentration : 1x10e6 cells per tests.
  Antibody Concentration Used: 0.5 μg/10e6 cells.
  Isotypic Control (shaded): FITC Mouse IgG2b.