C1QA Mouse Monoclonal Antibody [Clone ID: JL-1]
C1QA mouse monoclonal antibody, clone JL-1, Biotin
|Applications||ELISA, FN, IF, IHC, WB|
|Recommended Dilution||Immunohistochemistry on Frozen Sections (Ref.1): Stains tissue sections which were fixed in acetone. As positive control a polyclonal anti-C1q antibody was used and as negative control an isotype matched monoclonal antibody (Ref.1). The typical starting working dilution is 1/50.
Functional assays (Ref.1): Antibody JL-1 was administered to mice resulting in depletion of circulating C1q, glomerular deposition of C1q and induction of anti-C1q autoantibodies in susceptible mice. As a negative control an isotype matched monoclonal antibody was used (Ref.1).
Western blot (Ref.3): The typical starting working dilution is 1/50.
Positive Control: Spleen and kidney tissue of wild-type mice (Ref.1).
Negative Control: Spleen and kidney tissue of C1q -/- mice (Ref.1).
|Reactivities||Human, Mouse, Rat|
|Immunogen||Purified mouse C1q|
The monoclonal antibody JL-1 recognizes the collagen-like region (CLR) of mouse C1q.
State: Liquid 0.2 µm filtered Ig fraction
Stabilizer: 0.1% BSA
Preservative: 0.02% Sodium Azide
|Purification||Protein G Chromatography|
|Storage||Store undiluted at 2-8°C.
DO NOT FREEZE!
|Stability||Shelf life: one year from despatch.|
C1q is a 459 kDa molecule consisting of three individual polypeptide chains. The antibody has been generated by immunization of C1q-/- C57BL/6 mice with purified mouse C1q.
|Synonyms||Complement C1q, Complement 1q|