Leptin Receptor (LEPR) (soluble) Mouse Monoclonal Antibody [Clone ID: 2F1]

CAT#: AM26359PU-N

Leptin Receptor (LEPR) (soluble) mouse monoclonal antibody, clone 2F1, Purified

Specifications

Product Data

• Clone Name: 2F1
• Applications: ELISA, IHC, WB
• Recommended Dilution: Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
  Immunohistochemistry on paraffin sections: The typical starting working dilution is 1:50.
  Immunoassays (coating): The typical starting working dilution is 1:50.
  Western blot: The typical starting working dilution is 1:50.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity:
  The monoclonal antibody 2F1 reacts with human soluble leptin receptor (sLR) in plasma. It specifically reacts with sLR with a molecular mass of 180kD. The antibody can be used to measure in both free sLR and sLR bound to leptin in plasma.
• Formulation: PBS
  State: Purified
  State: Liquid Ig fraction
  Stabilizer: 0.1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: lot specific
• Purification: Protein G
• Conjugation: Unconjugated
• Storage:

  Store at 2 - 8 °C.
  

• Stability: Shelf life: one year from despatch.

• Gene Name: leptin receptor
• Database Link:
  Entrez Gene 3953 Human
  UniProt ID P48357
• Background: Leptin is a cytokine that is primarily expressed by adipose tissue. Leptin controls food intake by its interaction with the leptin receptor in the brain. Leptin action is mediated and controlled by the leptin receptor, a class I type cytokine receptor. sLR is generated by proteolytic cleavage of membrane-anchored receptors. This indicates that the leptin receptor might have other functions besides transduction. Binding of leptin with sLR increases the bioavailability of leptin in plasma, but also decreases the binding of leptin to membrane bound leptin receptors. For example, when comparing obese and lean individuals, plasma levels of sLR are significantly decreased whereas leptin levels are significantly increased.
• Synonyms: LEP-R, OB receptor, HuB219, LEPR, DB, OBR, OB-R