Lipoteichoic Acid (LTA) Mouse Monoclonal Antibody [Clone ID: 55]
Lipoteichoic Acid (LTA) mouse monoclonal antibody, clone 55, Low Endotoxin
|Applications||ELISA, IF, IHC, WB|
|Recommended Dilution||Immunohistochemistry on Frozen Sections: The typical starting working dilution is 1/50.
Flow Cytometry: 1/250 (Ref.10).
Immunoassay: For detection, 1.2 µg/ml antibody in PBS was added for 1 hr at 37°C on LTA coated plates (Ref.2,3,8).
Immunofluorescence (Ref.4,9): 60' in PBS/0.02%BSA/0.02% Saponin (Ref.4).
Western blot (Ref 5-7): A reduced or native sample treatment and run on 15% SDS-Page.
Blot was incubated o/n at 4°C with a 1/1000 dilution. The band size is ~17 kDa (Ref.6,7).
The typical starting working dilution is 1/50.
Positive Control: Culture medium of Gram-positive bacteria.
Negative Control: Culture medium of eukaryotic cells.
|Immunogen||Microbial mixture of Streptococcus sobrims HG961, HG962, HG970, and HG977 (Ref.1).|
The monoclonal antibody 55 recognizes Lipoteichoic Acid (LTA).
|Formulation||State: Low Endotoxin
State: Liquid Culture Medium with a Low Endotoxin level
Preservative: 0.02% Sodium Azide
|Storage||Store undiluted at 2-8°C.
DO NOT FREEZE!
|Stability||Shelf life: one year from despatch.|
|Background||LTA, a glycerol phosphate surface polymer, is a component of the envelope of Gram-positive bacteria. LTA is anchored via its glycolipids to the membrane and carries a polysaccharide chain extending into the peptidoglycan layer of the cell wall. LTA is released spontaneously into the culture medium during growth of gram-positive bacteria. LTA functions as an immune activator with characteristics very similar to lipopolysaccharide (LPS) from Gram-negative bacteria. LTA binds to CD14 and triggers activation predominantly via Toll-like receptor 2. Although LTA is internalized and traffics to the Golgi, the cellular activation in response to LTA occurs at the cell surface.|