Lipoteichoic Acid (LTA) Mouse Monoclonal Antibody [Clone ID: 55]

CAT#: AM26274LE-N

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Lipoteichoic Acid (LTA) mouse monoclonal antibody, clone 55, Low Endotoxin



USD 596.00

2 Weeks*

Size
    • 200 ug

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Specifications

Product Data
Clone Name 55
Applications ELISA, IF, IHC, WB
Recommended Dilution Immunohistochemistry on Frozen Sections: The typical starting working dilution is 1/50.
Flow Cytometry: 1/250 (Ref.10).
Immunoassay: For detection, 1.2 µg/ml antibody in PBS was added for 1 hr at 37°C on LTA coated plates (Ref.2,3,8).
Immunofluorescence (Ref.4,9): 60' in PBS/0.02%BSA/0.02% Saponin (Ref.4).
Western blot (Ref 5-7): A reduced or native sample treatment and run on 15% SDS-Page.
Blot was incubated o/n at 4°C with a 1/1000 dilution. The band size is ~17 kDa (Ref.6,7).
The typical starting working dilution is 1/50.
Positive Control: Culture medium of Gram-positive bacteria.
Negative Control: Culture medium of eukaryotic cells.
Reactivities Streptococcus sobrims
Host Mouse
Isotype IgG3
Clonality Monoclonal
Immunogen Microbial mixture of Streptococcus sobrims HG961, HG962, HG970, and HG977 (Ref.1).
Specificity

The monoclonal antibody 55 recognizes Lipoteichoic Acid (LTA).

Formulation State: Low Endotoxin
State: Liquid Culture Medium with a Low Endotoxin level
Preservative: 0.02% Sodium Azide
Concentration lot specific
Conjugation Unconjugated
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Stability Shelf life: one year from despatch.
Background LTA, a glycerol phosphate surface polymer, is a component of the envelope of Gram-positive bacteria. LTA is anchored via its glycolipids to the membrane and carries a polysaccharide chain extending into the peptidoglycan layer of the cell wall. LTA is released spontaneously into the culture medium during growth of gram-positive bacteria. LTA functions as an immune activator with characteristics very similar to lipopolysaccharide (LPS) from Gram-negative bacteria. LTA binds to CD14 and triggers activation predominantly via Toll-like receptor 2.  Although LTA is internalized and traffics to the Golgi, the cellular activation in response to LTA occurs at the cell surface.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.