GEF H1 (ARHGEF2) Mouse Monoclonal Antibody [Clone ID: B4/7]

CAT#: AM26229PU-N

GEF H1 (ARHGEF2) mouse monoclonal antibody, clone B4/7, Purified

Specifications

Product Data

• Clone Name: B4/7
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Immunohistochemstry on frozen sections (1): Cells were either fixed with methanol at -20 °C or with 3% PFA followed by permeabilization with Triton X-100. The typical starting working dilution is 1:50.
  Immunoflourescence (1-4).
  Immunprecipitation (1-3).
  Western blot (1-4): A non-reduced and/or reduced sample treatment and 6-15 % gradient SDS-PAGE was used. The band size is 110 kDa (Ref1-3). The typical starting working dilution is 1:50.
  Positive control: MDCK cells.
• Reactivities: Canine, Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: The monoclonal antibody B4/7 recognizes guanine nucleotide exchange factor H1 (GEF-H1).
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: lot specific
• Purification: Protein G
• Conjugation: Unconjugated
• Storage:

  Store at 2 - 8 °C.
  

• Stability: Shelf life: one year from despatch.

• Gene Name: Rho/Rac guanine nucleotide exchange factor 2
• Database Link:
  Entrez Gene 9181 Human
  UniProt ID Q92974
• Background:

  GEFH1 is an ~110 kDa protein belonging to the Dbl family of proto-oncogenes. GEF-H1 can activate the small GTPase RhoA, but not Rac1 or Cdc42. Rho family GTPases are central regulators of epithelial tight junctions and the cytoskeleton. GEF-H1 can associate with different cytoskeletal structures, namely microtubules and the actin cytoskeleton. It has also been proposed to mediate crosstalk between the two types of filaments.
  Localization of GEF-H1 differs between cell types. In MRC-5 fibroblast cells, GEF-H1 localizes to stress fibers. In epithelial cells, GEF-H1 is associated with apical tight junctions and involved in regulating paracellular permeability of small hydrophilic tracers. Furthermore, its subcellular localization changes in mitotic cells, where endogenous GEF-H1 is concentrated at mitotic spindles. GEF-H1 is capable of binding to the F-actin binding junctional adaptor cingulin. Binding to cingulin inhibits GEF-H1 and results in the downregulation of RhoA and inhibition of G1/S phase transition. In low confluent cultured cells, the localization of GEF-H1 is predominantly cytoplasmic. With increasing density of the cells, free GEF-H1 is sequestered at the tight junctions by cingulin.
  GEF-H1 is part of the signaling pathway connecting epithelial polarity with the cell cycle, and as such involved in oncogenesis.

• Synonyms: KIAA0651, LFP40, GEF-H1