HPV18 E7 Protein (36-70) Mouse Monoclonal Antibody [Clone ID: 8E2]

CAT#: AM26116PU-N

HPV18 E7 Protein (36-70) mouse monoclonal antibody, clone 8E2, Aff - Purified

Product Images

Western blot analysis of HPV-18 E7 protein. WB was carried out with 8E2 Mab using the lysates of U20S, Hela and SiHa cells. Line 1. U20S with plasmid expressing E7 protein, Line 2. plain U2OS cells. Lines 3 and 4. Lysate of Hela and SiHa cells accordingly.

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  IF analysis of HPV-18 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV-18 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus-localized HPV-18 E7 detected by Mab 8E2. IF analys

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  IP analysis of HPV-18 E7 protein. 1: detection of E7 protein by Mab 8E2 in lysate of U20S cells. 2: the lower band is precipitated E7, upper bands are light and heavy chain of Mab. 3: precipitation without mab 8E2. 4: IP exp. with plain U2OS cells. 5: IP e

Specifications

Product Data

• Clone Name: 8E2
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: Immunoflourescence: 0.33-10 µg/ml
  Immunoprecipitation (using recombinant HPV-18 E7- protein): 1:500
  ELISA (using recombinant HPV-18 E7- protein): 1:128 000.
  Western blot (using recombinant HPV-18 E7- protein): 1:1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant full-length HPV-18 E7-protein purified from E. coli
• Specificity: This antibody detects HPV18 E7 Protein at aa 36-70.
• Formulation: PBS pH 7.4, with 0.09% sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20 to -70 °C. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P06788
• Background: Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
• Synonyms: Human Papilloma Virus Early protein 7