Myosin Light Chain 2 (MYL2) (1-166) Mouse Monoclonal Antibody [Clone ID: AT3B2]

SKU AM09080PU-S

Myosin Light Chain 2 (MYL2) (1-166) mouse monoclonal antibody, clone AT3B2, Purified

Product Data

• Clone Name: AT3B2
• Application: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA.; Western blot (1/1,000-1/2,000).; Immunohistochemistry on Paraffin Sections (5 µg/ml). This MYL2 antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.; Immunocytochemistry / Immunoflourescence.; Flow Cytometry.
• Reactivity: Human, Rat
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Human MYL2 (1-166 aa) purified from E. coli
• Specificity: The antibody recognizes MYL2 (1-166).
• Buffer: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol; State: Purified; State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: myosin light chain 2
• Database Link:
  P10916
• Background: Myosin light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
• Synonyms: MLC-2v, MLC-2

Reference Data

• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction