H2-D1 Mouse Monoclonal Antibody [Clone ID: 27-11-13S]

CAT#: AM08082BT-N

H2-D1 mouse monoclonal antibody, clone 27-11-13S, Biotin

Specifications

Product Data

• Clone Name: 27-11-13S
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1-3)
  Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections only).
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C3H alphaBDF1 splenocytes. (Ref.1)
• Specificity: This antibody recognizes the alpha 3 domain of H-2Db class I MHC antigen. It cross-reacts with the alpha 3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd.
  Reactivity with haplotypes k, f, p, r, and s has not been observed.
  Customer feedback: Clone 27-11-13S does not cross-react with human cells.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: Biotin
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: histocompatibility 2, D region locus 1
• Database Link:
  Entrez Gene 14964 Mouse
  UniProt ID P01899
• Background: In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen).
  MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant beta 2 Microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
• Synonyms: H-2D(B), H2-D1