Cd28 Hamster Monoclonal Antibody [Clone ID: 37.51]
Cd28 hamster monoclonal antibody, clone 37.51, Purified
|Applications||FC, FN, IP|
|Recommended Dilution||Flow Cytometry (Ref.1,2).
Functional Assay: In vitro costimulation of T cells and NK cells (Ref.1-3,7-9,11).
|Immunogen||C57BL/6N mouse T cell lymphoma EL-4 cell line.|
|Specificity||This antibody is specific to CD28 costimulatory receptor (Mr. 40 kDa)|
|Formulation||Borate Buffered Saline, pH 8.2.
No preservatives or amine-containing buffer salts added.
State: Liquid purified Ig fraction.
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Gene Name||CD28 antigen|
|Background||CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. (Ref.1-4)
CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses. (Ref.5-8)