Cd28 Hamster Monoclonal Antibody [Clone ID: 37.51]

CAT#: AM08037PU-N

Cd28 hamster monoclonal antibody, clone 37.51, Purified

Specifications

Product Data

• Clone Name: 37.51
• Applications: FC, FN, IP
• Recommended Dilution: Flow Cytometry (Ref.1,2).
  Immunoprecipitation (Ref.1).
  Functional Assay: In vitro costimulation of T cells and NK cells (Ref.1-3,7-9,11).
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG2
• Clonality: Monoclonal
• Immunogen: C57BL/6N mouse T cell lymphoma EL-4 cell line.
• Specificity: This antibody is specific to CD28 costimulatory receptor (Mr. 40 kDa)
• Formulation: Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD28 antigen
• Database Link:
  Entrez Gene 12487 Mouse
  UniProt ID P31041
• Background: CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4⁺ and CD8⁺ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. (Ref.1-4)
  CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses. (Ref.5-8)
• Synonyms: TP44